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Enhancement of tropane alkaloids production in transgenic hair roots of Atropa belladonna by overexpressing endogenous genes AbPMT and AbH6H / 药学学报
Acta Pharmaceutica Sinica ; (12): 243-249, 2013.
Article in Chinese | WPRIM | ID: wpr-235677
Responsible library: WPRO
ABSTRACT
Atropa belladonna L. is the officially medicinal plant species and the main commercial source of scopolamine and hyoscyamine in China. In this study, we reported the simultaneous overexpression of two functional genes involved in biosynthesis of scopolamine, which respectively encoded the upstream key enzyme putrescine N-methyltransferase (PMT; EC 2.1.1.53) and the downstream key enzyme hyoscyamine 6beta-hydroxylase (H6H; EC 1.14.11.11) in transgenic hair root cultures of Atropa belladonna L. HPLC results suggested that four transgenic hair root lines produced higher content of scopolamine at different levels compared with nontransgenic hair root cultures. And scopolamine content increased to 8.2 fold in transgenic line PH2 compared with that of control line; and the other four transgenic lines showed an increase of scopolamine compared with the control. Two of the transgenic hair root lines produced higher levels of tropane alkaloids, and the content increased to 2.7 fold in transgenic line PH2 compared with the control. The gene expression profile indicated that both PMT and H6H expressed at a different levels in different transgenic hair root lines, which would be helpful for biosynthesis of scopolamine. Our studies suggested that overexpression of A. belladonna endogenous genes PMT and H6H could enhance tropane alkaloid biosynthesis.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Plants, Medicinal / Scopolamine / Tropanes / Atropa belladonna / Gene Expression Regulation, Enzymologic / Plants, Genetically Modified / Plant Roots / Gene Expression Regulation, Plant / Synthetic Biology / Hyoscyamine Language: Chinese Journal: Acta Pharmaceutica Sinica Year: 2013 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Plants, Medicinal / Scopolamine / Tropanes / Atropa belladonna / Gene Expression Regulation, Enzymologic / Plants, Genetically Modified / Plant Roots / Gene Expression Regulation, Plant / Synthetic Biology / Hyoscyamine Language: Chinese Journal: Acta Pharmaceutica Sinica Year: 2013 Type: Article