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Cloning and expression analysis of a key device of HMGR gene involved in ginsenoside biosynthesis of Panax ginseng via synthetic biology approach / 药学学报
Acta Pharmaceutica Sinica ; (12): 219-227, 2013.
Article in Chinese | WPRIM | ID: wpr-235680
ABSTRACT
3-Hydroxy-3-methylglutaryl coenzyme-A reductase (HMGR), the first enzyme of mavalonic acid pathway, is one of the key devices involved in ginsenoside biosynthesis based on synthetic biology approach. The open reading frame of a novel HMGR gene from Panax ginseng (PgHMGR2) was cloned and analyzed in this study. PgHMGR2-encoding protein showed 71.6% sequence similarity to a P. ginseng HMGR in GenBank. The full-length cDNA sequence of PgHMGR2 containing 1 770 bp, which encodes 589 amino acids, was cloned by RT-PCR strategy from P. ginseng. The bioinformatic analysis showed that PgHMGR2-encoding protein contained two transmembrane regions and the HMG_CoA_reductase domain, without signal peptide. The protein sequence of PgHMGR2 had the highest sequence similarity (99%) with Panax quinquefolius HMGR (GenBank accession No. ACV65036). The expression level of PgHMGR2 was the highest in flower based on a real-time PCR analysis, followed by leaf and root, and the lowest was in stem. The result will provide a foundation for exploring the molecular function of PgHMGR2 involved in ginsenoside biosynthesis based on synthetic biology approach in P. ginseng plants.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Phylogeny / Plants, Medicinal / Gene Expression Regulation, Enzymologic / Open Reading Frames / Amino Acid Sequence / Cloning, Molecular / Sequence Homology, Amino Acid / Protein Structure, Secondary / DNA, Complementary / Plant Stems Language: Chinese Journal: Acta Pharmaceutica Sinica Year: 2013 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Phylogeny / Plants, Medicinal / Gene Expression Regulation, Enzymologic / Open Reading Frames / Amino Acid Sequence / Cloning, Molecular / Sequence Homology, Amino Acid / Protein Structure, Secondary / DNA, Complementary / Plant Stems Language: Chinese Journal: Acta Pharmaceutica Sinica Year: 2013 Type: Article