Evaluation and comparison of native and recombinant LipL21 protein-based ELISAs for diagnosis of bovine leptospirosis
Journal of Veterinary Science
;
: 99-101, 2012.
Article
in English
| WPRIM
| ID: wpr-23570
ABSTRACT
A 21-kDa leptospiral lipoprotein (LipL21) was evaluated for its diagnostic potential to detect bovine leptospirosis by ELISA. Both native LipL21 (nLipL21) and recombinant LipL21 (rLipL21) proteins were tested and compared regarding diagnostic efficiency, and no statistically significant difference was observed. The sensitivity of rLipL21 ELISA for 62 microscopic agglutination test (MAT) positive sera was 100% and the specificity with 378 MAT negative sera was 97.09%. Thus, rLipL21 protein-based ELISA could be used as an alternative to MAT for the diagnosis of bovine leptospirosis.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Bacterial Outer Membrane Proteins
/
Recombinant Proteins
/
Enzyme-Linked Immunosorbent Assay
/
Cattle Diseases
/
Sensitivity and Specificity
/
Leptospira interrogans
/
Leptospirosis
/
Lipoproteins
/
Antibodies, Bacterial
/
Antigens, Bacterial
Type of study:
Diagnostic study
Limits:
Animals
Language:
English
Journal:
Journal of Veterinary Science
Year:
2012
Type:
Article
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