Evaluation and comparison of native and recombinant LipL21 protein-based ELISAs for diagnosis of bovine leptospirosis

Siju JOSEPH; Naicy THOMAS; E THANGAPANDIAN; Vijendra-P SINGH; Rishendra VERMA; S-K SRIVASTAVA

Siju JOSEPH; Naicy THOMAS; E THANGAPANDIAN; Vijendra-P SINGH; Rishendra VERMA; S-K SRIVASTAVA.

Journal of Veterinary Science ; : 99-101, 2012.

Article in English | WPRIM | ID: wpr-23570

ABSTRACT

ABSTRACT

A 21-kDa leptospiral lipoprotein (LipL21) was evaluated for its diagnostic potential to detect bovine leptospirosis by ELISA. Both native LipL21 (nLipL21) and recombinant LipL21 (rLipL21) proteins were tested and compared regarding diagnostic efficiency, and no statistically significant difference was observed. The sensitivity of rLipL21 ELISA for 62 microscopic agglutination test (MAT) positive sera was 100% and the specificity with 378 MAT negative sera was 97.09%. Thus, rLipL21 protein-based ELISA could be used as an alternative to MAT for the diagnosis of bovine leptospirosis.

Subject(s)

Animals; Cattle; Antibodies, Bacterial/blood; Antigens, Bacterial/biosynthesis; Bacterial Outer Membrane Proteins/biosynthesis; Cattle Diseases/blood; Enzyme-Linked Immunosorbent Assay/veterinary; Leptospira interrogans/isolation & purification; Leptospirosis/blood; Lipoproteins/biosynthesis; Recombinant Proteins/biosynthesis; Sensitivity and Specificity

ELISA; leptospirosis; microscopic agglutination test; nLipL21; rLipL21

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Bacterial Outer Membrane Proteins / Recombinant Proteins / Enzyme-Linked Immunosorbent Assay / Cattle Diseases / Sensitivity and Specificity / Leptospira interrogans / Leptospirosis / Lipoproteins / Antibodies, Bacterial / Antigens, Bacterial Type of study: Diagnostic study Limits: Animals Language: English Journal: Journal of Veterinary Science Year: 2012 Type: Article

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