Effect of salvianolic acid B on TNF-α induced cerebral microcirculatory changes in a micro-invasive mouse model / 中华创伤杂志(英文版)
Chinese Journal of Traumatology
; (6): 85-93, 2016.
Article
in En
| WPRIM
| ID: wpr-235776
Responsible library:
WPRO
ABSTRACT
<p><b>PURPOSE</b>To investigate the effects of salvianolic acid B (SAB) on tumor necrosis factor a (TNF-α) induced alterations of cerebral microcirculation with a bone-abrading model.</p><p><b>METHODS</b>The influences of craniotomy model and bone-abrading model on cerebral microcirculation were compared. The bone-abrading method was used to detect the effects of intracerebroventricular application of 40 μg/kg·bw TNF-α on cerebral venular leakage of fluorescein isothiocyanate (FITC)- albulmin and the rolling and adhesion of leukocytes on venules with fluorescence tracer rhodamine 6G. The therapeutical effects of SAB on TNF-α induced microcirculatory alteration were observed, with continuous intravenous injection of 5 mg/kg·h SAB starting at 20 min before or 20 min after TNF-α administration, respectively. The expressions of CD11b/CD18 and CD62L in leukocytes were measured with flow cytometry. Immunohistochemical staining was also used to detect E-selectin and ICAM-1 expression in endothelial cells.</p><p><b>RESULTS</b>Compared with craniotomy method, the bone-abrading method preserved a higher erythrocyte velocity in cerebral venules and more opening capillaries. TNF-α intervention only caused responses of vascular hyperpermeability and leukocyte rolling on venular walls, without leukocyte adhesion and other hemodynamic changes. Pre- or post-SAB treatment attenuated those responses and suppressed the enhanced expressions of CD11b/CD18 and CD62L in leukocytes and E-selectin and ICAM-1 in endothelial cells induced by TNF-α.</p><p><b>CONCLUSIONS</b>The pre- and post-applications of SAB during TNF-α stimulation could suppress adhesive molecular expression and subsequently attenuate the increase of cerebral vascular permeability and leukocyte rolling.</p>
Full text:
1
Index:
WPRIM
Main subject:
Pharmacology
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Reference Values
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Benzofurans
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Blood Flow Velocity
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Random Allocation
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Cerebrovascular Circulation
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Tumor Necrosis Factor-alpha
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Intercellular Adhesion Molecule-1
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E-Selectin
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Craniotomy
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
Chinese Journal of Traumatology
Year:
2016
Type:
Article