Nanoparticles as a vaccine adjuvant of anti-idiotypic antibody against schistosomiasis / 中华医学杂志(英文版)
Chinese Medical Journal
;
(24): 83-87, 2004.
Article
in English
| WPRIM
| ID: wpr-235827
ABSTRACT
<p><b>BACKGROUND</b>The development of new adjuvants for human use has been the focus of attention. This study's aim is to explore the possibility of using nanoparticle Ca nanoparticles (CA) as a vaccine adjuvant of anti-idiotypic antibody NP30 against schistosomiasis and its protective mechanisms.</p><p><b>METHODS</b>Nanoparticle CA-NP30 conjugate (CA-NP30) was fabricated. BALB/c mice were immunized actively with CA-NP30 to evaluate its effects of protective immunity on mice. The serum levels of specific IgG, IgG1 and IgG2a antibodies against NP30 and the concentrations of IFN-gamma and IL-4 in supernatant of splenocytes were determined via ELISA.</p><p><b>RESULTS</b>Nanoparticle CA could enhance significantly the protective immunity of NP30 against infection of Schistosoma japonicum and the worm reduction rose from 36.0% (NP30 alone) to 52.6%. The serum levels of specific IgG, IgG1 and IgG2a antibodies against NP30 increased remarkably, as compared with those of the group immunized with NP30 alone. The concentration of IFN-gamma in supernatant of splenocyte was drastically elevated [the groups immunized with CA-NP30 and NP30 alone were (493.80 +/- 400.74) pg/ml and (39.03 +/- 39.58) pg/ml, respectively], but the concentration of IL-4 showed no significant difference from that of NP30 alone [(27.94 +/- 9.84) pg/ml vs (27.28 +/- 14.44) pg/ml].</p><p><b>CONCLUSIONS</b>Nanoparticle CA could act as a vaccine adjuvant of anti-idiotypic antibody NP30 against schistosomiasis. The mechanism could be that CA-NP30 enhances humoral and cellular immune responses in mice.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Schistosomiasis
/
Antibodies, Helminth
/
Vaccines
/
Adjuvants, Immunologic
/
Antibodies, Anti-Idiotypic
/
Nanotechnology
/
Allergy and Immunology
/
Mice, Inbred BALB C
Limits:
Animals
Language:
English
Journal:
Chinese Medical Journal
Year:
2004
Type:
Article
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