Effect of human semaphorin-3F gene transient transfection on the proliferation of tongue carcinoma cells / 中华口腔医学杂志
Chinese Journal of Stomatology
;
(12): 726-728, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-235998
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of semaphorin-3F (SEMA-3F) gene transient transfection on the proliferation of Tca8113 tongue carcinoma cells.</p><p><b>METHODS</b>After construction of a full-length SEMA3F expression vector, Tca8113 cells were transient transfected with pEGFP-N1-SEMA3F by Lipofectamine 2000. The expression of SEMA-3F gene was detected by RT-PCR The differences of the expression in the transfected cell groups, empty vector groups and un-transfected cell groups were compared. The survival rates were assayed by methyl thiazolyl tetrazolium (MTT) enzymatic labeling technique. Cell cycle were assayed by flow cytometer (FCM).</p><p><b>RESULTS</b>The gene was transfected into Tca8113 cells. High expression of SEMA-3F was successfully detected in the transfected cell groups after gene transfection. The cell cycle percentage of G1 stage decreased and S stage increased.</p><p><b>CONCLUSIONS</b>SEMA-3F gene transient transfection may inhibit the proliferation of Tca8113 cells.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Tongue Neoplasms
/
Carcinoma, Squamous Cell
/
Transfection
/
Cell Cycle
/
Cell Line, Tumor
/
Cell Proliferation
/
Genetic Vectors
/
Genetics
/
Membrane Proteins
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Stomatology
Year:
2007
Type:
Article
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