The measurement of the third-order branches of the mesenteric artery tone by microvascular ring technique / 中国应用生理学杂志

ABSTRACT

<p><b>OBJECTIVE</b>In our study, the function of the third-order branches of the mesentenc artery was measured by microvascular ring technique, which can be used to detect microvascular function in some disease related to microvascular dysfunction.</p><p><b>METHODS</b>Isolated, fixed, standardized and then activated the third-order branches of rat mesenteric artery. Microvascular tone was measured by systolic and diastolic drags respectively, with the help of DMT tension apparatus and PowerLab data acquisition system.</p><p><b>RESULTS</b>The third-order branches of rat mesenteric artery showed excellent response to vasoactive drugs. The contraction effect of norepinephrine (NE) reached 19 in mN. When acetylcholine (Ach) or sodium nitroprusside (SNP) of 10(9)-10(5)mol/L was added, vascular tones showed gradient drop 80% of maximal relaxation when adding ACh, while 95% of maximal relaxation when adding SNP.</p><p><b>CONCLUSION</b>The third-order branches of the mesenteric artery function was successfully detected by using microvascular ring technique.</p>