Your browser doesn't support javascript.
loading
Effect of Xuebijing injection on TLR4--NF-kappaB--IL-1beta pathway of myocardial hypoxia/reoxygenation in rats / 中国应用生理学杂志
Article in Chinese | WPRIM | ID: wpr-236386
Responsible library: WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the role of Xuebijing injection(XBJI, traditional Chinese medicine), in inhibiting TLR4--NF-kappaB--IL-1beta pathway of myocardial hypoxia/reoxygenation in rats.</p><p><b>METHODS</b>Thirty six male SD rats (280 +/- 30) g were randomly divided into six groups (n = 6) normal group (N group), balanced perfusion group (BP group), model group (M group), low dose XBJI group (XBJI(L) group), middle dose XBJI group (XBJI(M) group), high dose XBJI group (XBJI(H) group). By Langendorff isolated heart perfusion device to establish the model of myocardial hypoxia/reoxygenation in rats. ELISA was used to detect the concentration of interleukin-1beta (IL-1beta); Western blot was used to detect the expression of nuclear factor kappa B p65 (NF-kappaB p65) protein and toll like receptor 4 (TLR4) protein; and RT-PCR to determine the expression of NF-kappaB p65 mRNA and TLR4 mRNA;To observe microstructure changes of hypoxia/reoxygenation myocardial by light microscopy.</p><p><b>RESULTS</b>Compared with M group, the IL-1beta concentration, NF-kappaB p65 and TLR4 protein,NF-kappaB p65 and TLR4 mRNA of XBJIL group, XBJI(M) group, XBJI(H) group expression decreased in varying degrees,and decreased most obviously all in XBJI(M) group (P < 0.05, P < 0.01); Myocardical structural damage was serious in M group, and improved after treatment XBJI, the most obvious was the XBJI(M).</p><p><b>CONCLUSION</b>Different dose of XBJI can inhibit TLR4--NF-kappaB--IL-1beta signal transduction pathway and reduce several inflammatory reaction after myocardial hypoxia/reoxygenation injury, the 4 ml/100 ml of XBJI is the best.</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Pathology / Pharmacology / Rats / Male / RNA, Messenger / Drugs, Chinese Herbal / Reperfusion Injury / Signal Transduction / Rats, Sprague-Dawley / Drug Therapy Language: Chinese Journal: Chinese Journal of Applied Physiology Year: 2014 Type: Article

Similar

MEDLINE

...
LILACS

LIS

Full text: Available Index: WPRIM (Western Pacific) Main subject: Pathology / Pharmacology / Rats / Male / RNA, Messenger / Drugs, Chinese Herbal / Reperfusion Injury / Signal Transduction / Rats, Sprague-Dawley / Drug Therapy Language: Chinese Journal: Chinese Journal of Applied Physiology Year: 2014 Type: Article