Myocardial single photon emission tomography imaging of reporter gene expression in rabbits / 中华心血管病杂志
Chinese Journal of Cardiology
;
(12): 548-553, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-236457
ABSTRACT
<p><b>OBJECTIVE</b>To explore the feasibility of single photon emission computed tomography (SPECT) detection of heart reporter gene expression and observed the optimal transfecting titer and imaging time by using herpes simplex virus 1-thymidine kinase (HSV1-tk) as reporter gene and 131I-2'-fluoro-2'-deoxy-1-beta-D-arabinofuranosyl-5-iodouracil (131I-FIAU) as reporter probe in rabbit myocardium.</p><p><b>METHODS</b>The recombinant Ad-tk carrying HSV1-tk gene and adenovirus (Ad) as vector was constructed and intramyocardially injected to rabbits at various concentrations (1 x 10(9) pfu, 5 x 10(8) pfu, 1 x 10(8) pfu, 5 x 10(7) pfu, 1 x 10(7) pfu). Two days later, rabbits were injected with 600 microCi 131I-FIAU in ear-margin vein and then underwent SPECT myocardium imaging for detection of HSV1-tk expression at 6 h, 24 h, 48 h and 72 h after injection, rabbits with 1 x 10(9) pfu Ad-tk injection were imaged at 96 h and 120 h. Rabbits were sacrificed after imaging and the total myocardial 131I-FIAU accumulation was quantified in percent of injected dose per gram myocardium (% ID/g). The myocardial Ad-tk expression was determined with RT-PCR.</p><p><b>RESULTS</b>Reporter gene was detected by SPECT imaging in the injection site while not detected in the control myocardium and site remote from injection. RT-PCR results also evidenced HSV1-tk express in the injection site. The SPECT target/nontarget ratio was correlated with ex vivo gamma-counting (r2 = 0.933, P<0.01) and expression of HSV1-tk (r2 = 0.877, P<0.01). Myocardial accumulation could be identified at viral titers as low as 1 x 10(7) pfu by SPECT imaging.</p><p><b>CONCLUSION</b>The cardiac SPECT reporter gene imaging with HSV1-tk as reporter gene and 131I-FIAU as reporter probe is feasible.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Thymidine Kinase
/
Uracil
/
Diagnostic Imaging
/
Transfection
/
Gene Expression
/
Tomography, Emission-Computed, Single-Photon
/
Genes, Reporter
/
Gene Transfer Techniques
/
Genetics
/
Heart
Type of study:
Diagnostic study
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Cardiology
Year:
2009
Type:
Article
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