Cell attachment of periodontal ligament cells on commercially pure titanium at the early stage / 华中科技大学学报(医学)(英德文版)
Journal of Huazhong University of Science and Technology (Medical Sciences)
; (6): 307-310, 2004.
Article
in En
| WPRIM
| ID: wpr-236538
Responsible library:
WPRO
ABSTRACT
In order to study the character of periodontal ligament cells (PDLCs) attaching on commercially pure titanium (cpTi) by morphology and metrology on the early stage (24 h), 1 x 10(5)/ml PDLCs in 2 ml culture medium were seeded on cpTi discs fixed in 24-well culture plates. Morphology of cell attachment was observed by contrast phase microscope, scanning electron microscope (SEM) and fluroscence microscopy. Cell adhesion was analyzed by MTT at 0.5, 1, 2, 4 h respectively. PDLCs could attach and spread on cpTi discs. SEM showed that PDLCs had pseudopod-like protuberance. PDLCs showed different attaching phases and reached saturation in cell number at 2 h. It was concluded that PDLCs had good biocompatibility with cpTi, and showed a regular and dynamic pattern in the process of attaching to cpTi.
Full text:
1
Index:
WPRIM
Main subject:
Periodontal Ligament
/
Pharmacology
/
Surface Properties
/
Time Factors
/
Titanium
/
Biocompatible Materials
/
Dental Implants
/
Cell Adhesion
/
Cells, Cultured
/
Cell-Matrix Junctions
Limits:
Humans
Language:
En
Journal:
Journal of Huazhong University of Science and Technology (Medical Sciences)
Year:
2004
Type:
Article