Cloning of integral mature peptide gene of human GDF-5 / 华中科技大学学报(医学)(英德文版)
Journal of Huazhong University of Science and Technology (Medical Sciences)
;
(6): 212-213, 2004.
Article
in English
| WPRIM
| ID: wpr-236570
ABSTRACT
The integral mature peptide gene of human growth differentiation factor-5 (GDF-5) was cloned to provide the essential foundation for study on the biological characteristics of GDF-5 at gene and protein levels. Two primers were chemosynthesized according to the hGDF-5 sequence reported in Genbank. The hGDF-5 gene was gained by RT-PCR methods from the total RNA extracted from human fetus cartilage tissue, and was cloned into vector pMD18-T. The sequence of recombinant plasmid pMD18-T-hGDF-5 was analyzed by sequence analysis. DNA agarose gel electrophoresis showed that the product of RT-PCR was about 380bp, and double enzyme digestion of the recombinant plasmid corresponded with it. The result of sequence assay was in agreement with the reported hGDF-5 sequence in Genbank. Our results showed that the integral mature peptide gene of human GDF-5 was cloned successfully from human fetal cartilage tissue, and totally identified with the sequence of human GDF-5 in Genbank.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Cartilage
/
Chemistry
/
Transforming Growth Factor beta
/
Cloning, Molecular
/
Bone Morphogenetic Proteins
/
Growth Differentiation Factor 5
/
Fetus
/
Genetic Vectors
/
Genetics
Type of study:
Prognostic study
Limits:
Humans
Language:
English
Journal:
Journal of Huazhong University of Science and Technology (Medical Sciences)
Year:
2004
Type:
Article
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