Construction and identification of immortalized rat astrocyte cell line expressing enkephalin / 中华创伤杂志(英文版)
Chinese Journal of Traumatology
;
(6): 353-356, 2007.
Article
in English
| WPRIM
| ID: wpr-236751
ABSTRACT
<p><b>OBJECTIVE</b>To provide a sound cell source for further ex-vivo gene therapy for chronic pain, we attempt to develop an immortalized rat astrocyte cell line that expresses enkephalin regulated by doxycycline.</p><p><b>METHODS</b>Retrovirus infection method was employed to develop an immortalized rat astrocyte cell line that could express enkephalin regulated by doxycycline. The hPPE gene expression level of immoralized astroyte cells (IAC)/ hPPE was detected by RT-PCR, indirect immunofluorescence staining and radioimmunoassay.</p><p><b>RESULTS</b>IAC carrying Tet-on system transfected with preproenkephalin gene could secrete enkephalin that was regulated by doxycycline in a dose-dependent manner and hPPE gene activation could be repeated in on-off-on cycles through administration or removal of doxycycline.</p><p><b>CONCLUSION</b>An immortalized rat astrocyte cell line that secrete enkephalin under the control of doxycycline is established successfully, which provides a research basis for transgenic cell transplantation for analgesia.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Protein Precursors
/
Retroviridae
/
Enkephalins
/
Cell Line, Transformed
/
Genetic Therapy
/
Astrocytes
/
Chronic Disease
/
Doxycycline
/
Neurotransmitter Agents
Limits:
Animals
Language:
English
Journal:
Chinese Journal of Traumatology
Year:
2007
Type:
Article
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