Proteomic analysis of paclitaxel-induced apoptosis in MCF-7 human breast carcinoma cells / 中华肿瘤杂志
Chinese Journal of Oncology
;
(12): 418-421, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-236927
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the mechanism of paclitaxel-induced apoptosis in MCF-7 human breast carcinoma cells.</p><p><b>METHODS</b>In this study, the proteins extracted from paclitaxel-induced apoptotic MCF-7 cells were analyzed by 2-dimentional gel electrophoresis (2-DE), and compared with those from untreated MCF-7 cells. The differential proteins were identified by mass spectrometry.</p><p><b>RESULTS</b>At 24 hour after paclitaxel (100 nmol/L) treatment, MCF-7 cells were collected and extracted the whole proteins. Seventeen up-regulated or down-regulated proteins were found by analysis of the differential proteomic 2-DE map. Six of them were identified by mass spectrometry. They were enolase 1, chloride intracellular channel 1, keratin 8, ribosomal protein S12, galectin-1 and histidine triad nucleotide binding protein, respectively.</p><p><b>CONCLUSION</b>We effectively found the changed proteins in the process of paclitaxel-induced apoptosis in MCF-7 human breast carcinoma cells by proteomic techniques. These up-regulated or down-regulated proteins are important molecules for our further research about the mechanism of paclitaxel-induced apoptosis.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Phosphopyruvate Hydratase
/
Ribosomal Proteins
/
Breast Neoplasms
/
Electrophoresis, Gel, Two-Dimensional
/
Paclitaxel
/
Apoptosis
/
Galectin 1
/
Proteomics
Limits:
Female
/
Humans
Language:
Chinese
Journal:
Chinese Journal of Oncology
Year:
2006
Type:
Article
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