Knocking down osteopontin expression by specific siRNA reduces the in vitro invasiveness of human hepatocellular carcinoma cells / 中华肿瘤杂志
Chinese Journal of Oncology
;
(12): 404-407, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-236930
ABSTRACT
<p><b>OBJECTIVE</b>To study the effect of osteopontin (OPN) expression down-regulated by RNA interference (RNAi) on the invasiveness of hepatocelluar carcinoma cell line HCC-LM3.</p><p><b>METHODS</b>HCC-LM3 cells were transfected with the chemically synthesized small interfering RNA (siRNA) formulated by lipofectamine 2000. Wild type HCC-LM3 and HCC-LM3 cells transfected with non-specific siRNA served as controls. Real-time PCR and Western blotting were used to quantify the mRNA and OPN protein levels. The malignant phenotypes of transfected HCC-LM3 cells including cellular growth rate, colony formation and Matrigel invasion activities were analyzed.</p><p><b>RESULTS</b>Sequence-specific siRNAs targeting OPN suppressed OPN RNA expression by 79% and also decreased OPN protein level by 81% in HCC-LM3 cells. The number of formed colonies and migrating numbers in vitro were decreased in HCC-LM3 cells transfected using sequence-specific siRNAs targeting OPN relative to controls (P < 0.05).</p><p><b>CONCLUSION</b>This study demonstrated that specific siRNA is able to reduce OPN at both the mRNA and protein levels and significantly diminishes the invasiveness of hepatocellular carcinoma cells.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
RNA, Messenger
/
Transfection
/
Down-Regulation
/
Cell Movement
/
Carcinoma, Hepatocellular
/
RNA, Small Interfering
/
RNA Interference
/
Cell Line, Tumor
/
Cell Proliferation
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Oncology
Year:
2006
Type:
Article
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