Enhancement of HER-2 degradation by carbamazepine in breast cancer SKBR-3 cell line / 中华肿瘤杂志
Chinese Journal of Oncology
;
(12): 503-506, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-236948
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the anti-tumor effect and the mechanism of down-regulation of HER - 2 by cabamazepine in SKBR - 3 cells , a breast cancer cell line with HER - 2 over - expression.</p><p><b>METHODS</b>Western blotting was performed to evaluate the Her-2 expression level. The mRNA level of HER-2 was detected by RT-PCR. Immunoprecipitation was applied to detect the chaperon function and acetylation level of HSP90. The viability of cells was tested by MTT assay.</p><p><b>RESULTS</b>Cabamazepine treatment down-regulated HER-2 expression. Only HER-2 protein level decrease was observed with 10 micromol/L cabamazepine treatment, but both protein and mRNA expressions were inhibited by 100 micromol/L cabamazepine. Cabamazepine treatment could induce a higher acetylation level of HSP90 and destroy its chaperon function. Cabamazepine exerted synergism with Herceptin in promoting HER-2 protein degradation and synergism or potentiation with Herceptin or 17-AAG in inhibition of proliferation.</p><p><b>CONCLUSION</b>Cabamazepine can reduce the expression of HER-2 and show a synergistic effect with Herceptin or 17-AAG. There may be potential benefits of carbamazepine for cancer therapy in future. HER-2;</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Acetylation
/
Breast Neoplasms
/
RNA, Messenger
/
Carbamazepine
/
Down-Regulation
/
Gene Expression Regulation, Neoplastic
/
Blotting, Western
/
Benzoquinones
Limits:
Female
/
Humans
Language:
Chinese
Journal:
Chinese Journal of Oncology
Year:
2006
Type:
Article
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