Introduce Tagsk1 into salt-sensitive callus to improve the capacity of salt-tolerance by micropartical bombardment / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 211-214, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-236999
ABSTRACT
The Tagsk1 (Triticum asetium L. glycogen synthase kinase 1) gene derived from the genome of wheat salt-tolerance mutant RH8706-49 was cloned by PCR. The special primers designed according to full length cDNA sequence of Tagsk1 (AF525086). A binary expression vector pBI121-gsk1 containing Gus and Tagsk1 was constructed. And pBI121-gsk1 was introduced into the callus induced from mature embryos of salt-sensitive wheat H8706-34 and cv. China Spring by particle bombardment. The transformed callus were screened by Kanamycin and 0.5% NaCl. The salt-tolerance callus were obtained, which showed higher ability of salt-tolerance and could diffirentiate roots and buds on the medium containing 0.5% NaCl.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Physiology
/
Plant Proteins
/
Seeds
/
Transformation, Genetic
/
Triticum
/
Adaptation, Physiological
/
Sodium Chloride
/
Plants, Genetically Modified
/
DNA, Plant
/
Biolistics
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2006
Type:
Article
Similar
MEDLINE
...
LILACS
LIS