Metabolism of recombinant CHO-GS cell reducing of toxic effect of ammonia / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 94-100, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-237018
ABSTRACT
The toxic effect of ammonia on rCHO-GS cell decreased obviously due to the transfection of GS system in serum-free culture. The maximum cell density, 15.6 x 10(5) cells/mL was obtained in the culture with 1.42 mmol/L ammonia. The growth of rCHO-GS cell was inhibited with an increased ammonia concentration. However, a cell density of 8.9 x 10(5) cells/mL was obtained when the concentration of ammonia was 12.65mmol/L. The intracellar metabolic pathways were affected due to the decrease of the toxic effect of ammonia on rCHO-GS cell. With the increase of initial ammonia concentration from 0.36mmol/L to 12.65mmol/L, the yield coefficients of cell to glucose and lactate to glucose decreased. The activities of hexokinase (HK), pyruvate kinase (PK), and lactate dehydrogenase (LDH) increased by 43%, 140% and 25%, respectively, indicating that the utilization of glucose increased and the glycolysis pathway was more prone to efficient energy metabolism pathway. An increased activity of glutamate-pyruvate aminotransferase (GPT) showed that the conversation from glutamate to alpha-ketoglutarate was shifted to glutamate-pyruvate transamination pathway. The deamination pathway was inhibited due to a decreased activity of glutamate dehydrogenase. In addition, the number of cells in G0/G1 phase increased and the specific production rate of recombinant protein increased by 2.1-fold with the increase of initial ammonia concentration from 0.36mmol/L to 12.65mmol/L.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Genetic Engineering
/
Cricetulus
/
Culture Media, Serum-Free
/
CHO Cells
/
Cell Culture Techniques
/
Toxicity
/
Genetics
/
Glutamine
/
Glutamate-Ammonia Ligase
/
Ammonia
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2006
Type:
Article
Similar
MEDLINE
...
LILACS
LIS