Isolation of BADH gene promoter from Suaeda liaotungensis and its sequence analysis / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 77-81, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-237021
ABSTRACT
In this study, the 5' -flanking proximal region of stress-induced gene encoding betaine aldehyde dehydrogenase was isolated by Adaptor-PCR and TAIL-PCR from halophyte Suaeda liaotungensis. 1993 bp sequence was obtained by sequencing. The transcription start site, which localized at 62 bases upstream of the start ATG, was predicted using TSSP-TCM program. The functional elements were analysed by PLACE programm. The SlBADH gene promoter contains the basic elements TATA-box, CAAT-box, and stress-induced elements salt responsed element, cold, dehydration, ABA and frozen responsed elements, WUN responsed elements and HSE. Obtaining the promoter of betaine aldehyde dehydrogenase gene from Suaeda liaotungensis provides a foundation for analyzing the stress-induced promoter elements, studying the relationship between structure and founction of the promoter, and investigating the molecular mechanism of BADH gene regulation.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Plant Proteins
/
Salts
/
Molecular Sequence Data
/
Base Sequence
/
Gene Expression Regulation, Enzymologic
/
Promoter Regions, Genetic
/
Sequence Analysis, DNA
/
Genes, Plant
/
Cold Temperature
/
Gene Expression Regulation, Plant
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2006
Type:
Article
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