Purification of a ginsenoside-Rb1 hydrolase from Helix snailase / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 929-933, 2005.
Article
in Chinese
| WPRIM
| ID: wpr-237048
ABSTRACT
Through a combination of twice DEAE chromatography by NaCl stepwise and gradient elution with gel filtration chromatography, a kind of ginsenoside-Rb1 hydrolase from crude Helix snailase was separated. The hydrolase was purified to apparent homogeneity on SDS-PAGE. It was estimated that the purified hydrolase was consisted of four identical subunits with a molecular mass of 110-115 kD by SDS-PAGE and gel filtration chromatography. The Km and Vmax values for ginsenoside-Rb1 were calculated to be 0.790 mmol/L and 10.192 micromol/(min x mg) of protein respectively. The ginsenoside-Rb1 hydrolase could only hydrolyze the glycosidic bond at the C20 position of ginsenoside-Rb1 into ginsenoside-Rd.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Catalysis
/
Chemistry
/
Ginsenosides
/
Helix, Snails
/
Hydrolases
/
Metabolism
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2005
Type:
Article
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