Deletions and rearrangements of PAX5 gene in B-lineage acute lymphoblastic leukemia / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
;
(6): 549-552, 2013.
Article
in Chinese
| WPRIM
| ID: wpr-237209
ABSTRACT
<p><b>OBJECTIVE</b>To determine the frequency paired-box domain 5 (PAX5) gene alterations in B-lineage acute lymphoblastic leukemia (B-ALL) harboring 9p abnormalities and its implication for clinical prognosis.</p><p><b>METHODS</b>Bacterial artificial chromosomes RP11-344B23 and RP11-652D9 encompassing the PAX5 gene were selected. DNA was extracted with conventional method and labeled with fluorescein by nicking transition. Fluorescence in situ hybridization (FISH) was used to determine the rearrangement or deletion of the PAX5 gene in B-ALL harboring chromosome 9p abnormalities. Clinical and laboratory features of patients were analyzed.</p><p><b>RESULTS</b>Fifty cases were analyzed with FISH. Complete deletion was observed in 23 patients (46%), partial deletion was observed in 2 patients (4%), and rearrangement was detected only in 1 case. The total frequency of abnormalities was 52% (26/50). No significant difference was found in clinical features of patients with or without PAX5 rearrangement or deletion.</p><p><b>CONCLUSION</b>The frequency of PAX5 gene alterations in B-ALL harboring 9p abnormalities was 52%. However, no significant difference was found between patients with and without PAX5 alterations.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Chromosomes, Human, Pair 9
/
Gene Rearrangement
/
Leukemia, B-Cell
/
Acute Disease
/
Sequence Deletion
/
In Situ Hybridization, Fluorescence
/
PAX5 Transcription Factor
/
Genetics
Type of study:
Prognostic study
Limits:
Adolescent
/
Adult
/
Child
/
Female
/
Humans
/
Male
Language:
Chinese
Journal:
Chinese Journal of Medical Genetics
Year:
2013
Type:
Article
Similar
MEDLINE
...
LILACS
LIS