Expression of FOS protein in glioma and its effect on the growth of human glioma cells / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
;
(6): 293-296, 2013.
Article
in Chinese
| WPRIM
| ID: wpr-237264
ABSTRACT
<p><b>OBJECTIVE</b>To study the expression of FOS protein in human glioma tissues and its effect on tumor growth.</p><p><b>METHODS</b>FOS protein expression in glioma tissues was determined with immunohistochemical (IHC) staining. Subsequently, 3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-diphenytetrazoliumromide (MTT) assay, flow cytometry, transwell invasion and Western blotting were used to assay U87 and U251 cells with reduced FOS expression.</p><p><b>RESULTS</b>The expression of FOS in glioma was increased and strongly correlated with its pathological grade. Abrogating expression of FOS has suppressed proliferation and invasion, and delayed cell cycle at G1 phrase for both U87 and U251 cells.</p><p><b>CONCLUSION</b>The expression of FOS protein in human glioma was strong. FOS protein probably plays a critical role in the progression of gliomas.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Gene Expression
/
Cell Movement
/
Proto-Oncogene Proteins c-fos
/
Cyclin D1
/
Matrix Metalloproteinase 9
/
RNA Interference
/
Cell Line, Tumor
/
Cell Proliferation
/
Neoplasm Grading
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Medical Genetics
Year:
2013
Type:
Article
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