Auto-mobilized adult hematopoietic stem cells advance neovasculature in diabetic retinopathy of mice / 中华医学杂志(英文版)
Chinese Medical Journal
;
(24): 2265-2268, 2010.
Article
in English
| WPRIM
| ID: wpr-237468
ABSTRACT
<p><b>BACKGROUND</b>Hematopoietic stem cells (HSCs) can be used to deliver functionally active angiostatic molecules to the retinal vasculature by targeting active astrocytes and may be useful in targeting pre-angiogenic retinal lesions. We sought to determine whether HSC mobilization can ameliorate early diabetic retinopathy in mice.</p><p><b>METHODS</b>Mice were devided into four groups normal mice control group, normal mice HSC-mobilized group, diabetic mice control group and diabetic mice HSC mobilized group. Murine stem cell growth factor (murine SCF) and recombined human granulocyte colony stimulating factor (rhG-csf) were administered to the mice with diabetes and without diabetes for continuous 5 days to induce autologous HSCs mobilization, and subcutaneous injection of physiological saline was used as control. Immunohistochemical double staining was conducted with anti-mouse rat CD31 monoclonal antibody and anti-BrdU rat antibody.</p><p><b>RESULTS</b>Marked HSCs clearly increased after SCF plus G-csf-mobilization. Non-mobilized diabetic mice showed more HSCs than normal mice (P=0.032), and peripheral blood significantly increased in both diabetic and normal mice (P=0.000). Diabetic mice showed more CD31 positive capillary vessels (P=0.000) and accelerated endothelial cell regeneration. Only diabetic HSC-mobilized mice expressed both BrdU and CD31 antigens in the endothelial cells of new capillaries.</p><p><b>CONCLUSION</b>Auto-mobilized adult hematopoietic stem cells advance neovasculature in diabetic retinopathy of mice.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Immunohistochemistry
/
Granulocyte Colony-Stimulating Factor
/
Hematopoietic Cell Growth Factors
/
Platelet Endothelial Cell Adhesion Molecule-1
/
Hematopoietic Stem Cell Mobilization
/
Therapeutic Uses
/
Diabetic Retinopathy
/
Drug Therapy
/
Metabolism
/
Methods
Limits:
Animals
/
Female
/
Humans
Language:
English
Journal:
Chinese Medical Journal
Year:
2010
Type:
Article
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