Erythropoietin gene-modified conditioned medium of human mesenchymal cells promotes hematopoietic development from human embryonic stem cells / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 976-980, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-237612
ABSTRACT
The study was aimed to investigate the effect of deriving hematopoietic cells from human embryonic stem cells (hESCs) by the erythropoietin gene-modified conditioned medium of human mesenchymal cells. The mesenchymal stem cells (MSCs) steadily expressing EPO were established by lentiviral system. The expression of exogenous EPO was detected by RT-PCR and Western blot. After suspension culture, hESCs developed into embryonic bodies (EBs). Then the EB cells were cultured in conditional medium. The hESCs-derived hematopoietic cells were analyzed by immunofluorescence, CFU assay and RT-PCR. The results indicated that the exogenous EPO successfully expressed in the EPO transfected MSCs (EPO/MSCs). The supernatant from EPO/MSCs increased CD34(+) cell population and the expression of globin, and enhanced colony forming unit incidence. These effects were obviously higher than that of control. It is concluded that the EPO gene-modified conditioned medium of human mesenchymal cells can induce the hESCs to differentiate into hematopoietic cells.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Cell Differentiation
/
Erythropoietin
/
Culture Media, Conditioned
/
Cell Culture Techniques
/
Organisms, Genetically Modified
/
Cell Biology
/
Embryonic Stem Cells
/
Mesenchymal Stem Cells
/
Genetics
Limits:
Humans
Language:
Chinese
Journal:
Journal of Experimental Hematology
Year:
2010
Type:
Article
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