Study of reduction rat T-lymphocytes activation and proliferation by inhibition inducible co-stimulator gene expression / 中华外科杂志
Chinese Journal of Surgery
;
(12): 301-304, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-237797
ABSTRACT
<p><b>OBJECTIVE</b>To observe the change of activation and proliferation ability of rat T-lymphocytes after suppress ICOS gene expression by RNA interference.</p><p><b>METHODS</b>Four interference sites targeting at rat ICOS gene were designed and four pairs of oligonucleotide fragments were cloned into the pSilencer 4.1-CMV neo plasmid vectors then transfected into rat lymphocytes with cationic liposome. The expression of mRNA and protein of ICOS was detected by RT-PCR and flow cytometry. The alteration of lymphocyte proliferation ability was evaluated by mix lymphocyte reaction, and the secretion levels of IFN-gamma and IL-4 were measured by ELISA procedure.</p><p><b>RESULTS</b>After transfection, the expression of mRNA and protein of ICOS in test groups were lower than that in control groups (P < 0.05). The ability of T-lymphocytes in proliferation was poor and the levels of IFN-gamma and IL-4 were reduced with ICOS gene shut down.</p><p><b>CONCLUSIONS</b>RNA interference plasmid vector can suppress ICOS expression in rat T-lymphocytes significantly, and it may be useful for further study on transplantation immunity tolerance.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Lymphocyte Activation
/
T-Lymphocytes
/
Transfection
/
Antigens, Differentiation, T-Lymphocyte
/
Cells, Cultured
/
Interleukin-4
/
Interferon-gamma
/
Rats, Sprague-Dawley
/
RNA Interference
/
Cell Proliferation
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Surgery
Year:
2008
Type:
Article
Similar
MEDLINE
...
LILACS
LIS