Molecular cloning and identification of calmegin gene in embryo and adult testes by cDNA microarray hybridization / 中华男科学杂志
National Journal of Andrology
;
(12): 355-360, 2003.
Article
in Chinese
| WPRIM
| ID: wpr-238025
ABSTRACT
<p><b>OBJECTIVE</b>To identify gene differential expression in embryo and adult testes by cDNA microarray techniques.</p><p><b>METHODS</b>cDNA probes of embryo and adult testes were used to hybridize the cDNA microarray of human testis, and the clones of differential hybridization were sequenced and analyzed.</p><p><b>RESULTS</b>The calmegin (CLGN) gene which had been confirmed to be involved in spermatogenesis was found, and it exhibited more than 20-fold difference at expression level between adult and embryo human testes.</p><p><b>CONCLUSION</b>Genes differential expression in adult and embryo human testes can be identified by cDNA microarray hybridization.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Spermatogenesis
/
Testis
/
Calcium-Binding Proteins
/
Molecular Sequence Data
/
Base Sequence
/
Chemistry
/
Embryology
/
Cloning, Molecular
/
Molecular Chaperones
/
Gene Expression Regulation, Developmental
Limits:
Adult
/
Humans
/
Male
Language:
Chinese
Journal:
National Journal of Andrology
Year:
2003
Type:
Article
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