Effects of triptolide on anti-angiogenesis by reducing expression of urokinase plasminogen activator / 生物医学工程学杂志
Journal of Biomedical Engineering
;
(6): 778-781, 2005.
Article
in Chinese
| WPRIM
| ID: wpr-238343
ABSTRACT
In order to unveil the anti-angiogenic mechanism of triptolide, we investigated the effects of triptolide on the proliferation, migration, angiogenesis and u-PA expression of cultured HUVECs. MTT assay found that triptolide could inhibit the proliferation of HUVECs, and three-dimensional culture system revealed that triptolide could curb the migration of HUVECs. The chick embryo chorioallantoic membrane (CAM) test showed that triptolide could inhibit angiogenesis. Real time quantitive RT-PCR showed the expression of u-PA of HUVECs was down-regulated by triptolide. Therefore, triptolide may inhibit the proliferation and migration of endothelial cell by way of reducing the expression of u-PA. These findings may conduce to the elucidation of the anti-angiogenic mechanism of triptolide.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Phenanthrenes
/
Umbilical Veins
/
RNA, Messenger
/
Urokinase-Type Plasminogen Activator
/
Down-Regulation
/
Cells, Cultured
/
Angiogenesis Inhibitors
/
Cell Biology
/
Endothelial Cells
Limits:
Animals
/
Humans
Language:
Chinese
Journal:
Journal of Biomedical Engineering
Year:
2005
Type:
Article
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