Nitroglycerin tolerance aggravates arterial ischemia/reperfusion injury by increasing nitrotyrosine and ONOO- production / 中华心血管病杂志
Chinese Journal of Cardiology
;
(12): 680-684, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-238540
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the influence of nitroglycerin tolerance (NT) on arterial ischemia (90 min) and reperfusion (120 min).</p><p><b>METHODS</b>Male Sprague-Dawley rats were infused with nitroglycerin (GTN) or saline for 12 h and ascending aorta was rapidly isolated. The isolated aorta was subjected to one of the following treatments stimulative ischemia/reperfusion, stimulative ischemia/reperfusion (I/R) plus glutathione (GSH, 0.1 mmol/L) during reperfusion, or control solution (Kreb's solution for 3.5 h).</p><p><b>RESULTS</b>Compared with I/R group, contractile function, vasorelaxation responses to Ach, NO production were significantly decreased and CK, LDH activity as well as nitrotyrosine formation in reperfusion solution were significantly increased in I/R + NT group and these effects could be prevented with addition of GSH in I/R + NT aortas.</p><p><b>CONCLUSIONS</b>Our results demonstrated that NT could aggravate arterial I/R injury by increasing the production of ONOO- and GSH may play a cardioprotective role against NT-induced myocardial injury by attenuating the formation of ONOO-.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Aorta
/
Pathology
/
Pharmacology
/
Vasoconstriction
/
In Vitro Techniques
/
Reperfusion Injury
/
Nitroglycerin
/
Rats, Sprague-Dawley
/
Drug Tolerance
/
Glutathione
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Cardiology
Year:
2006
Type:
Article
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