C-reactive protein induced human endothelium cells apoptosis is associated with Bcl-2/Bax gene expression changes / 中华心血管病杂志
Chinese Journal of Cardiology
;
(12): 639-642, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-238546
ABSTRACT
<p><b>OBJECTIVE</b>In the present study, we examined the expression changes of Bcl-2/Bax in C-reactive protein (CRP) treated human endothelium cells in vitro.</p><p><b>METHODS</b>The human umbilical vein endothelial cells (HUVEC) were cultured by digest method for 2 - 3 posterities and incubated with human CRP (0, 1, 5, 25 mg/L for 24 hours) and analyzed by flow cytometer for apoptosis ratio. The effects of CRP in various concentrations on Bcl-2/Bax mRNA and protein expression were examined by RT-PCR and Western Blotting.</p><p><b>RESULTS</b>Apoptosis ratio increased, downregulated Bcl-2 (gene promoting cell survival) and upregulated Bax (gene promoting apoptosis) at mRNA and protein levels in proportion to increased CRP concentrations.</p><p><b>CONCLUSION</b>These results demonstrate that Bcl-2/Bax could be regulated by CRP in human HUVECs and might play a causal role in CRP-induced apoptosis.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Umbilical Veins
/
C-Reactive Protein
/
Cell Line
/
Apoptosis
/
Proto-Oncogene Proteins c-bcl-2
/
Reverse Transcriptase Polymerase Chain Reaction
/
Cell Biology
/
Endothelial Cells
/
Bcl-2-Associated X Protein
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Cardiology
Year:
2006
Type:
Article
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