Endothelial progenitor cells related gene expression changes before and early after revascularization in patients with acute myocardial infarction / 中华心血管病杂志

ABSTRACT

<p><b>OBJECTIVE</b>The purpose of this study was to observe the endothelial progenitor cells (EPCs) related gene expression changes before and early after revascularization in patients with acute myocardial infarction.</p><p><b>METHODS</b>Peripheral blood samples were taken from patients with acute anterior myocardial infarction 6 hours and 7 days after PCI and stenting. Mononuclear cells (MNCs) were isolated by Ficoll-density centrifugation and cultured in M-199 medium. After 14 days culture, attaching cells incorporated DiI-acetylated low-density lipoprotein (EPCs) were collected and RNA was isolated by Trizol for microarray analysis on 24 genes associated with permissibility/vessel tone (angiotensin system ACE, AGTR-1, AGTR-2; NO system eNOS; prostacyclin system COX-2; endothelin system ET-1, ETA, ETB; superoxide anions system SOD-1), angiogenesis (adhesion molecule CDH5; growth factors and receptors VEGFR1, VEGFR2, VEGF) and endothelial cell activation (adhesion molecules expression ICAM1, ICAM2, ICAM3, PECAM-1, E-Selectin, L-Selection, VCAM1; change phenotype from antithrombotic to prothrombotic tPA, uPA, PAI, vWF). VEGFR2, PECAM-1 and VE-cadherin positive cells were identified by flow cytometry.</p><p><b>RESULT</b>Eight gene expressions (AGTR-1, AGTR-2, COX-2, eNOS, ET-1, ETA, VEGF) were significantly downregulated 7 days post PCI compared to pre-PCI (P < 0.05). Flow cytometry results showed that VEGFR2 positive cells were also significantly reduced post PCI than that of before PCI (P < 0.05).</p><p><b>CONCLUSION</b>PCI down-regulated endothelial progenitor cells related gene expressions in patients with acute myocardial infarction.</p>