Determination of eight ginsenosides in renshenshouwu capsules by HPLC-DAD / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To establish an HPLC-DAD method for simultaneous determination of eight ginsenosides in Renshenshouwu capsules.</p><p><b>METHOD</b>Ultimate C18 column (4.6 mm x 250 mm, 5 microm) was adopted for gradient elution, with acetonitrile and water as the mobile phase. The flow rate was 1 mL x min(-1), the column temperature was set at 30 degrees C, and the detection wavelength was 203 nm.</p><p><b>RESULT</b>A good linearity was observed in the range of 0.242-12.1, 0.222-11.1, 0.251-25.1, 0. 245-24.5, 0.232-23.2, 0.232-23.2, 0.264-26.4, 0.244-24.4 microg for ginsenoside Rg1, Re, Rb1, Rc, Rb2, Rb3, Rd and 20(S)-ginsenoside Rg3, respectively, with the average recoveries of 102.7%, 103.2%, 101.6%, 101.2%, 102.0%, 100.7%, 101.9%, 102.0%, respectively.</p><p><b>CONCLUSION</b>The method was so simple, accurate and effective that it could be used for quality control of the above eight components in Renshenshouwu capsules.</p>