Human osteoblasts response to different magnitudes of mechanical stimulation in vitro / 中华外科杂志
Chinese Journal of Surgery
;
(12): 381-384, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-238888
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of different magnitudes of tensile strain on human osteoblasts differentiation.</p><p><b>METHODS</b>According to the strain amplification mechanism at cellular level and a data calculated by finite element analysis, the cellular level strain of 0.8%, 1.6%, 2.4% and 3.2% was respectively applied to human osteoblasts for 48 h at a frequency of 1 Hz. Alkaline phosphatase activity and the expression of osteoblast-related genes were detected by Semi-Quantitative RT-PCR and densitometric analysis.</p><p><b>RESULTS</b>Alkaline phosphatase activity significantly increased at 0.8% and 1.6%. The level of osteocalcin mRNA increased at 2.4% and 3.2%. Cbfa1/Runx2 gene expression only increased at 3.2%. Comparing to static control, mRNA level of type I collagen increased at every magnitude. The mRNA level decreased at 0.8% and increased at 3.2% when compared to the group with 1.6% elongation.</p><p><b>CONCLUSIONS</b>Higher magnitudes of strain enhance expression of osteocalcin, type I collagen gene and Cbfa1/Runx2 in human osteoblasts, but lost the ability to increase ALP activity which is remained by lower magnitudes of strain. Type I collagen gene expression increases in a strain magnitude dependent manner.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Osteoblasts
/
Stress, Mechanical
/
Osteocalcin
/
Cell Line
/
Gene Expression Regulation
/
Cell Biology
/
Collagen Type I
/
Cell Proliferation
/
Alkaline Phosphatase
/
Core Binding Factor Alpha 1 Subunit
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Surgery
Year:
2009
Type:
Article
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