The effect of rosiglitazone on the activity of STAT1 in rats with severe acute pancreatitis / 中华外科杂志
Chinese Journal of Surgery
;
(12): 218-221, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-238922
ABSTRACT
<p><b>OBJECTIVE</b>To explore the effect of rosiglitazone on the activity of signal transducer and activator of transcription 1 in rats with severe acute pancreatitis.</p><p><b>METHODS</b>Fifty-four male Wistar rats were randomly allocated into three groups (n = 18). SO group sham-operated animals served as control, operation was executed and sodium chloride but not sodium taurocholate was injected. SAP group SAP was induced by the retrograde injection of 5% sodium taurocholate into the biliopancreatic duct. ROSI group same as SAP group, but rosiglitazone (6 mg/kg) was administered intravenously 30 min before operation. Rats in each group were sacrificed at 3,6 and 12 h after operation. The levels of serum amylase and histologic scores of pancreatic tissue were measured. The expression of TNF-alpha mRNA in pancreatic tissue were determined by reverse transcription-polymerase chain reaction (RT-PCR). The expression of phosphorylated STAT1 in pancreatic tissue was assayed by immunohistochemistry.</p><p><b>RESULTS</b>Compared to SO group, the levels of serum amylase and phosphorylated STAT1, TNF-alpha mRNA and histologic scores of pancreatic tissue were significantly elevated at the same time points after SAP (P < 0.01). The levels of these detection in ROSI group were lower than those of the SAP group at the same time points (P < 0.05), but higher than SO group (P < 0.05).</p><p><b>CONCLUSIONS</b>STAT1 was activated in severe acute pancreatitis. Rosiglitazone has a protective effects in rats with severe acute pancreatitis. The mechanism of its protective effects maybe that it inhibits the activation of JAK/STAT pathway, which can down-regulate the expression of TNF-alpha mRNA and block the the inflammatory cascade partially.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pancreas
/
Pancreatitis
/
Pathology
/
Pharmacology
/
Random Allocation
/
Acute Disease
/
Rats, Wistar
/
Thiazolidinediones
/
Disease Models, Animal
/
Drug Therapy
Type of study:
Prognostic study
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Surgery
Year:
2009
Type:
Article
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