Expression of macrophage inflammatory protein-1alpha in EPS and its significance / 中华男科学杂志
National Journal of Andrology
;
(12): 63-66, 2012.
Article
in Chinese
| WPRIM
| ID: wpr-239006
ABSTRACT
<p><b>OBJECTIVE</b>To detect the mRNA and protein expressions of MIP-1alpha in EPS and determine their significance in the sub-typing of chronic prostatitis.</p><p><b>METHODS</b>We collected samples of expressed prostatic secretion (EPS) from 50 cases of chronic prostatitis, including 16 cases of chronic bacterial prostatitis (CBP), 23 cases of chronic nonbacterial prostatitis/chronic pelvic pain syndrome (CPPS) (11 CPPS IIIA, 12 CPPS IIIB), and 11 cases of type-IV asymptomatic inflammatory prostatitis (AIP). Another 15 healthy volunteers were included as normal controls. The mRNA and protein levels of MIP-1alpha in EPS were determined by RT-PCR and ELISA, respectively, followed by statistical analysis with SPSS 15.0.</p><p><b>RESULTS</b>The mRNA expression of MIP-1alpha was markedly higher in the CPPS IIIA and CPPS IIIB groups than in the others (P<0.05). The protein level of MIP-1alpha was (1174.3 +/- 89.2) pg/ml in CPPS IIIA and (842.3 +/- 76.2) pg/ml in CPPS IIIB, significantly higher than (198.0 +/- 37.8) pg/ml in the control, (347.0 +/- 61.6) pg/ml in CBP and (292.0 +/- 56.4) pg/ml in type-IV AIP (P<0.05).</p><p><b>CONCLUSION</b>Determination of mRNA and protein levels of MIP-1alpha in EPS may help the sub-typing and diagnosis of chronic prostatitis.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Prostate
/
Prostatitis
/
Body Fluids
/
Case-Control Studies
/
Chronic Disease
/
Bodily Secretions
/
Chemokine CCL3
/
Metabolism
Type of study:
Observational study
Limits:
Adult
/
Aged
/
Humans
/
Male
Language:
Chinese
Journal:
National Journal of Andrology
Year:
2012
Type:
Article
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