Gossypol decreases the expression of connexin 43 in Sertoli cells / 中华男科学杂志
National Journal of Andrology
;
(12): 993-997, 2011.
Article
in Chinese
| WPRIM
| ID: wpr-239045
ABSTRACT
<p><b>OBJECTIVE</b>To study the effect of gossypol on the expression of connexin 43 (CX43) in Sertoli cells.</p><p><b>METHODS</b>TM4 Sertoli cells were cultured and treated with gossypol at the concentrations of 1.25, 2.5, 5 and 10 micromol/L for 6, 12, 24 and 48 hours. The cytotoxicity of gossypol was assessed by CCK-8 assay, and the expression of CX43 in the normal TM4 Sertoli cells and in those treated with different concentrations of gossypol for different times was detected by RT-PCR and immunofluorescence analysis.</p><p><b>RESULTS</b>Semiquantitative RT-PCR and immunofluorescence analysis showed the expression of CX43 in the normal TM4 cells. At 24 hours of exposure to gossypol, the expression began to decrease gradually with the prolonging of time and the increasing concentration of gossypol (P < 0.05).</p><p><b>CONCLUSION</b>Gossypol reduces the expression of CX43 in TM4 Sertoli cells, which might underlie the mechanism of its antifertility action.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Sertoli Cells
/
Gossypol
/
Cells, Cultured
/
Connexin 43
/
Toxicity
/
Metabolism
Limits:
Humans
/
Male
Language:
Chinese
Journal:
National Journal of Andrology
Year:
2011
Type:
Article
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