Evaluation of three methods for forensic diatom test / 南方医科大学学报
Journal of Southern Medical University
;
(12): 427-431, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-239163
ABSTRACT
<p><b>OBJECTIVE</b>To compare the efficacy of three methods for forensic diatom test, namely strong acid digestion-centrifuge enrichment-light microscopy (SD-CE-LM), microwave digestion-membrane filtration-automated scanning electron microscopy (MD-ME-SEM), and microwave digestion-membrane filtration-light microscopy (MD-MF-LM).</p><p><b>METHODS</b>Sixty samples were randomly divided into 3 groups for diatom test using three methods, and the sample preparation time, degree of digestion and recovery rate of diatoms were compared.</p><p><b>RESULTS</b>The sample preparation time was the shortest with MD-MF-LM and the longest with SD-CE-LM (P<0.05). MD-ME-SEM and MD-MF-LM allowed more thorough tissue digestion than SD-CE-LM. MD-ME-SEM resulted in the highest total recovery rate of diatom, followed by MD-MF-LM and then by SD-CE-LM (P<0.05); the recover rate of different diatom species was the highest with MD-ME-SEM, followed by MD-MF-LM and SD-CE-LM (P<0.05).</p><p><b>CONCLUSION</b>SD-CE-LM has a low recovery rate of diatoms especially for those with lengths shorter than 40 µm or densities less than 1/5. With a high recovery rate and accuracy in diatom test, MD-ME-SEM is suitable for diagnosis of suspected drowning cases. MD-MF-LM is highly efficient, sensitive and convenient for forensic diatom test.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Specimen Handling
/
Microscopy, Electron, Scanning
/
Centrifugation
/
Diatoms
/
Forensic Sciences
/
Drowning
/
Methods
/
Microscopy
/
Microwaves
Limits:
Humans
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2015
Type:
Article
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