Adenovirus vector-mediated short hairpin RNA targeting nuclear factor-κB suppresses proliferation of endometrial cells of Macaca fascicularis in vitro / 南方医科大学学报
Journal of Southern Medical University
;
(12): 390-396, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-239170
ABSTRACT
<p><b>OBJECTIVE</b>To assess the effect of a high specific adenovirus vector-mediated shRNA targeting nuclear factor-κB (NF-κB) on cell proliferation of the endometrium of Macaca fascicularis.</p><p><b>METHODS</b>The adenoviral vector NF-κB-p65-shRNA and the empty vector were separately trasnfected in cultured endometrial cells of Macaca fascicularis. The changes in the expression of the target gene protein and apoptotic proteins, cell proliferation, and cell cycle distribution were observed after the transfection.</p><p><b>RESULTS</b>Compared with the control cells, infection of the endometrial cells with the NF-κB-p65-shRNA adenovirus significantly increased the expression levels of apoptotic proteins, promoted apoptosis of the endometrial cells, and reduced the cells in division?stage.</p><p><b>CONCLUSIONS</b>NF-κB-p65 shRNA adenovirus can effectively promote apoptosis of endometrial cells and inhibit the proliferation of endometrial cells of Macaca fascicularis.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Transfection
/
Cells, Cultured
/
Adenoviridae
/
Apoptosis
/
Cell Biology
/
RNA, Small Interfering
/
Cell Proliferation
/
Endometrium
/
Transcription Factor RelA
/
Genetic Vectors
Limits:
Animals
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2015
Type:
Article
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