Preparation and characterization of uricase in uricase-catalase liposomes prepared using borate buffer / 南方医科大学学报
Journal of Southern Medical University
;
(12): 268-271, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-239198
ABSTRACT
<p><b>OBJECTIVE</b>To characterize the property of uricase loaded in uricase-catalase liposomes (BUCLPs) prepared using borate buffer.</p><p><b>METHODS</b>BUCLPs were prepared using reverse-phase evaporation, and the physicochemical properties of uricase in the prepared BUCLPs were examined.</p><p><b>RESULTS</b>The optimal temperature of BUCLP and URI was 40 degrees celsius, their optimal pH values were 8.0 and 8.5, and their Michaelis-Menten constants were 14.207 µmol/L and 13.623 µmol/L, respectively. Fluorescence intensity of nanoliposome-loaded uricase-catalase that bound to FITC was higher than that of uricase-catalase binding directly with FITC; the fluorescence intensity of BUCLP was higher than that of free uricase-catalase at 280 nm.</p><p><b>CONCLUSION</b>Uricase activity is enhanced after loading in uricase and catalase liposomes.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Temperature
/
Urate Oxidase
/
Borates
/
Catalase
/
Chemistry
/
Nanoparticles
/
Liposomes
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2015
Type:
Article
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