Src kinase inhibitor PP2 protects rat astrocytes from hypoxia/reoxygenation injury in vitro / 南方医科大学学报
Journal of Southern Medical University
;
(12): 239-243, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-239205
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of Src kinase inhibitor PP2 on hypoxia/reoxygenation (H/R) injury in rat astrocytes in vitro.</p><p><b>METHODS</b>In vitro cultured rat astrocytes were exposed to hypoxia for 8 h followed by reoxygenation for 24 h with or without pretreatment with PP2 (10 µmol/L) for 24 h before H/R injury. MTT assay and flow cytometry were used to detect the viability and apoptosis of the exposed astrocytes, respectively, and the protein expressions of Src, Bax, and Bcl-2 in the cells were determined using Western blotting.</p><p><b>RESULTS</b>PP2 pretreatment significantly increased the viability and decreased the apoptosis rate of rat astrocytes exposed to H/R injury (P<0.01). Western blotting showed that H/R injury caused increased expression of Src kinase, which was lowered by PP2 pretreatment. The ratio of Bax/bcl-2 in the astrocytes increased after H/R injury, and was significantly decreased by PP2 pretreatment (P<0.01).</p><p><b>CONCLUSION</b>PP2 protects rat astrocytes from H/R injury possibly by inhibiting the expression of Src kinase and activating the anti-apoptotic mechanisms in the cells.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Pyrimidines
/
Cell Hypoxia
/
Cells, Cultured
/
Astrocytes
/
Apoptosis
/
Src-Family Kinases
/
Flow Cytometry
Limits:
Animals
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2015
Type:
Article
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