Effects of HCV core protein on the expression of hypoxia-inducible factor 1 alpha and vascular endothelial growth factor / 中华肝脏病杂志
Chinese Journal of Hepatology
;
(12): 751-754, 2011.
Article
in Chinese
| WPRIM
| ID: wpr-239334
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of core protein of hepatitis C virus (HCV) on the expression of hypoxia-inducible factor-1alpha (HIF-1alpha) and vascular endothelial growth factor (VEGF).</p><p><b>METHODS</b>Huh7.5.1 cells were transfected with plasmid flag2B-core carrying HCV core gene, expression of HIF-1alpha and VEGF were measured by reverse transcription-polymorphism chain reaction (RT-PCR) and western blot. Enzyme link immunoabsorbent assay (ELISA) were used to detect the level of VEGF in the supernatants.</p><p><b>RESULTS</b>The expression of HIF-1alpha and VEGF mRNA and protein were upregulated after flag2B-core was transfected into Huh7.5.1 cells, and VEGF level in the supernatant was significant elevated as compared to controls [(654.5+/-43.7) pg/ml vs (365.9+/-26.8) pg/ml, t = 653.1%, P less than 0.01]. The expression of HIF-1alpha and VEGF mRNA and protein were downregulated after flag2B-core and HIF-1alpha siRNA were co-transfected into Huh7.5.1 cells, and VEGF level in the supernatant was significantly reduced as compared to controls [(389.2+/-29.6) pg/ml vs (768.8+/-47.3)pg/ml, t = 1330.22, P less than 0.01].</p><p><b>CONCLUSIONS</b>HCV core protein enhances the expression of HIF-1alpha and VEGF. HCV may regulate the expression of HIF-1alpha and VEGF via the core protein.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Transfection
/
Viral Core Proteins
/
RNA, Small Interfering
/
Cell Line, Tumor
/
Vascular Endothelial Growth Factor A
/
Hypoxia-Inducible Factor 1, alpha Subunit
/
Genetics
/
Metabolism
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Hepatology
Year:
2011
Type:
Article
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