The qualitative analysis and quantitative analysis of purification of salvianolic acids by macroreticular resin / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To purify salvianolic acids by macroreticular resin,then mensurate the contents of salvianolic acids and analyse the chromatogram with HPLC.</p><p><b>METHOD</b>Make salvianolic acids with macroreticular resin; mensurate the content of Salvianolic acids with UV spestrophotometry the control compound is protocaechuic aldehyde, and the wavelength is 281 nm. Analysis the chromatogram with HPLC, and compare the chromatogram in different technics zorbax ODS column (4.6 mm x 250 mm, 5 microm), mobilephase 1% aceticacid-water and methanol in different proportions, the wavelength is 281 nm.</p><p><b>RESULT</b>The contents of salvianolic acids is 53.8%; HPLC chromatogram indicate that the method is reasonable to make salvianolic acids.</p><p><b>CONCLUSION</b>Determination of contents and HPLC chromatogram can control the quality of Salvianolic acids more accurately.</p>