Chemoresistance of CD133+ cancer stem cells in laryngeal carcinoma / 中华医学杂志(英文版)
Chinese Medical Journal
;
(24): 1055-1060, 2011.
Article
in English
| WPRIM
| ID: wpr-239894
ABSTRACT
<p><b>BACKGROUND</b>Mounting evidence suggests that tumors are histologically heterogeneous and are maintained by a small population of tumor cells termed cancer stem cells. CD133 has been identified as a candidate marker of cancer stem cells in laryngeal carcinoma. This study aimed to analyze the chemoresistance of CD133(+) cancer stem cells.</p><p><b>METHODS</b>The response of Hep-2 cells to different chemotherapeutic agents was investigated and the expression of CD133 was studied. Fluorescence-activated cell sorting analysis was used to identify CD133, and the CD133(+) subset of cells was separated and analyzed in colony formation assays, cell invasion assays, chemotherapy resistance studies, and analyzed for the expression of the drug resistance gene ABCG2.</p><p><b>RESULTS</b>About 1% - 2% of Hep-2 cells were CD133(+) cells, and the CD133(+) proportion was enriched by chemotherapy. CD133(+) cancer stem cells exhibited higher potential for clonogenicity and invasion, and were more resistant to chemotherapy. This resistance was correlated with higher expression of ABCG2.</p><p><b>CONCLUSIONS</b>This study suggested that CD133(+) cancer stem cells are more resistant to chemotherapy. The expression of ABCG2 could be partially responsible for this. Targeting this small population of CD133(+) cancer stem cells could be a strategy to develop more effective treatments for laryngeal carcinoma.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Peptides
/
Pharmacology
/
Neoplastic Stem Cells
/
Carcinoma
/
Glycoproteins
/
Antigens, CD
/
Laryngeal Neoplasms
/
Blotting, Western
/
Cisplatin
/
Paclitaxel
Limits:
Humans
Language:
English
Journal:
Chinese Medical Journal
Year:
2011
Type:
Article
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