Expression of PML-RAR alpha fusion transcripts detection by using RQ-PCR / 中华血液学杂志
Chinese Journal of Hematology
; (12): 753-756, 2008.
Article
in Zh
| WPRIM
| ID: wpr-239961
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To establish the real time quantitative PCR (RQ-PCR) assay according to 'Europe Against Cancer' (EAC) program and analyze the results of detection and quantification of different PML-RAR alpha transcript isoforms in patients with acute promyelocytic leukemia (APL).</p><p><b>METHODS</b>Three RQ-PCR systems were performed to detect the most frequent PML-RAR alpha transcripts (L-form, S-form and V-form) in 30 APL patients and the RQ-PCR end-point products were identified by electrophoresis.</p><p><b>RESULTS</b>S-form RQ-PCR system could amplify positive signals of three isoforms in all of 30 cases, and V-form RQ-PCR system could do so in both L-form and V-form positive cases, however, L-form RQ-PCR system could only do so in L-form-positive cases. Electrophoresis and sequencing of end-point products amplified by S-form RQ-PCR system revealed three bands in each of L-form (621 bp, 477 bp and 218 bp) and V-form (567 bp, 423 bp and 218 bp) positive patients samples.</p><p><b>CONCLUSIONS</b>RQ-PCR, sensitive and reliable, can be used for monitoring the minimal residual disease in APL patients, however, its results should be interpreted carefully if it is used for detection of PML-RAR alpha fusion transcripts prospectively.</p>
Full text:
1
Index:
WPRIM
Main subject:
Leukemia, Promyelocytic, Acute
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Oncogene Proteins, Fusion
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Sensitivity and Specificity
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Neoplasm, Residual
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Reverse Transcriptase Polymerase Chain Reaction
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Diagnosis
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Genetics
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Methods
Type of study:
Diagnostic_studies
Limits:
Adolescent
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Adult
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Child
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Female
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Humans
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Male
Language:
Zh
Journal:
Chinese Journal of Hematology
Year:
2008
Type:
Article