Influence of gene transduction mediated by lentivirus vector on gene expression of human CD34+ cord blood cells / 中华血液学杂志
Chinese Journal of Hematology
;
(12): 393-396, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-240006
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the influence of gene transduction mediated by lentivirus vector on human CD34+ cord blood cell (CBCs) gene expression.</p><p><b>METHODS</b>CD34+ cells were isolated and transduced with the third-generation self-inactivating ( SIN) lentiviral vector carrying green fluorescent protein (GFP). The total RNA from transduced cells was extracted and the differences of genotypes between the transduced and non-transduced CD34+ cells were determined with cDNA microarray analysis.</p><p><b>RESULTS</b>In 23000 genes two were upregulated and six downregulated. These changes were not confirmed by semi-quantitative RT-PCR method.</p><p><b>CONCLUSIONS</b>Lentiviral vector used in this study do not influence significantly on the gene expression of CD34+ CBCs, and the vector system may be a useful and safe one in clinical gene therapy.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Transduction, Genetic
/
Transfection
/
Cells, Cultured
/
Lentivirus
/
Antigens, CD34
/
Oligonucleotide Array Sequence Analysis
/
Gene Expression Profiling
/
Cell Biology
/
Fetal Blood
/
Genetic Vectors
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Hematology
Year:
2008
Type:
Article
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