Effect of tetrandrine in combination with droloxifen on the expression of NF-kappaB protein in K562 and K562/A02 cell lines / 中华血液学杂志
Chinese Journal of Hematology
; (12): 321-324, 2008.
Article
in Zh
| WPRIM
| ID: wpr-240017
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the effect of tetrandrine (Tet) in combination with droloxifen (DRL) on the expression of nuclear factor kappa B (NF-kappaB) in K562 and K562/A02 cell lines and its reversal mechanism.</p><p><b>METHODS</b>The activation of NF-kappaB in K562 and K562/A02 cell lines and the effect of Tet or DRL alone or in combination on NF-kappaB protein expression were determined with immunocytochemistry and Western blotting respectively.</p><p><b>RESULTS</b>(1) K562/A02 cells displayed higher level of NF-kappaB protein expression than K562 cells. (2) The application of Tet or DRL alone or in combination had no effect on NF-kappaB expression in K562 cells at 6 h and 12 h (P > 0.05). (3) Tet and DRL alone or in combination could significantly down-regulate NF-kappaB protein expression in nuclei of K562/A02 cells. The effect was more significant in combination than either alone. This effect was more significant at 12 h than at 6 h.</p><p><b>CONCLUSIONS</b>(1) Activation of NF-kappaB may be involved in the mechanism of MDR of K562/A02 cell line. (2)Inhibition of NF-kappaB activation may be involved in the reversal of multidrug resistance in K562/A02 cells by Tet and DRL.</p>
Full text:
1
Index:
WPRIM
Main subject:
Pharmacology
/
Tamoxifen
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NF-kappa B
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Drug Resistance, Multiple
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K562 Cells
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Benzylisoquinolines
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Metabolism
Limits:
Humans
Language:
Zh
Journal:
Chinese Journal of Hematology
Year:
2008
Type:
Article