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Current advances in diagnostic methods of Acanthamoeba keratitis / 中华医学杂志(英文版)
Chinese Medical Journal ; (24): 3165-3170, 2014.
Article in English | WPRIM | ID: wpr-240210
ABSTRACT
<p><b>OBJECTIVE</b>The objective of this article was to review the current advances in diagnostic methods for Acanthamoeba keratitis (AK).</p><p><b>DATA SOURCES</b>Data used in this review were retrieved from PubMed (1970-2013). The terms "Acanthamoeba keratitis" and "diagnosis" were used for the literature search.</p><p><b>STUDY SELECTION</b>Data from published articles regarding AK and diagnosis in clinical trials were identified and reviewed.</p><p><b>RESULTS</b>The diagnostic methods for the eight species implicated in AK were reviewed. Among all diagnostic procedures, corneal scraping and smear examination was an essential diagnostic method. Polymerase chain reaction was the most sensitive and accurate detection method. Culturing of Acanthamoeba was a reliable method for final diagnosis of AK. Confocal microscopy to detect Acanthamoeba was also effective, without any invasive procedure, and was helpful in the early diagnosis of AK.</p><p><b>CONCLUSION</b>Clinically, conjunction of various diagnostic methods to diagnose AK was necessary.</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Physiology / Visual Acuity / Acanthamoeba Keratitis / Diagnosis Type of study: Diagnostic study / Screening study Limits: Humans Language: English Journal: Chinese Medical Journal Year: 2014 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Physiology / Visual Acuity / Acanthamoeba Keratitis / Diagnosis Type of study: Diagnostic study / Screening study Limits: Humans Language: English Journal: Chinese Medical Journal Year: 2014 Type: Article