Role of mitogen-activated protein kinases in the regulation of paraventricular nucleus to gastric ischemia-reperfusion injuries / 中华医学杂志(英文版)
Chinese Medical Journal
;
(24): 1082-1087, 2007.
Article
in English
| WPRIM
| ID: wpr-240267
ABSTRACT
<p><b>BACKGROUND</b>We investigated the role in electrical stimulations of paraventricular nucleus (PVN) on gastric mucosal cells and the activity of mitogen-activated protein kinases (MAPKs) family members induced by gastric ischemia-reperfusion (GI-R). And we elucidated the molecular mechanisms of the protection of PVN from GI-R injuries.</p><p><b>METHODS</b>Sprague-Dawley rats were divided randomly into 4 groups Group I, the sham-operated GI-R control group; Group II, the sham-operated electrical stimulations to PVN + sham-operated GI-R control group; Group III, the GI-R group; and Group IV, the electrical stimulations to PVN + GI-R group. In all of the experiments, the PVN was stimulated prior to the induction of GI-R. The GI-R model was established by clamping the celiac artery for 30 minutes to induce ischemia and then was released to allow reperfusion for 30 minutes, 1 hour, 3 hours and 6 hours, respectively. The gastric mucosal cellular apoptosis, proliferation, and the expression and activity of MAPKs protein were observed by immunohistochemistry and Western blotting, respectively.</p><p><b>RESULTS</b>Compared with the GI-R group, the application of electrical stimulations in the PVN significantly depressed gastric mucosal cellular apoptosis and enhanced gastric mucosal cellular proliferation following the 30-minute, 1-hour and 3-hour intervals of reperfusion; it also promoted the activation of p-ERK during the early phase of reperfusion but inhibited the activation of p-JNK1/2 and p-p38 following the 30-minute, 1-hour and 3-hour intervals of reperfusion.</p><p><b>CONCLUSIONS</b>The protection of PVN against GI-R injuries may attribute to the inhibition of apoptosis and the promotion of the proliferation of gastric mucosal cells during GI-R. This protective effect is mediated by activating the ERK pathway and depressing the JNK, p38 MAPK pathways of the gastric mucosal cells.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Paraventricular Hypothalamic Nucleus
/
Pathology
/
Phosphorylation
/
Physiology
/
Reperfusion Injury
/
Rats, Sprague-Dawley
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Apoptosis
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MAP Kinase Signaling System
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Extracellular Signal-Regulated MAP Kinases
/
JNK Mitogen-Activated Protein Kinases
Limits:
Animals
Language:
English
Journal:
Chinese Medical Journal
Year:
2007
Type:
Article
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