The influence of adrenaline on the expression of TGF-beta1, bFGF and I procollagen for hypertrophic scar / 中华整形外科杂志
Chinese Journal of Plastic Surgery
;
(6): 440-444, 2005.
Article
in Chinese
| WPRIM
| ID: wpr-240406
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the influence of adrenaline on the expression of TGFbeta1, bFGF and procollagen for human normal and hypertrophic scar dermal fibroblasts cultured in vitro.</p><p><b>METHODS</b>Human normal and hypertrophic scar dermal fibroblasts were propagated in a serum-free in vitro model with adrenaline for 24 hours. The human mRNA levels of bFGF, TGF-beta1 and I procollagen in fibroblasts were determined by RT-PCR. Levels of bFGF and TGF-beta1 in the supernatants of fibroblasts cultured in vitro were determined by enzyme-linked immunosorbent assay (ELISA).</p><p><b>RESULTS</b>In our study, adrenaline caused statistically significant increase in the peak levels of bFGF for normal and hypertrophic scar fibroblast cell lines (P < 0.01). It also caused statistically significant decrease in the level of TGF-beta1 for normal and hypertrophic scar fibroblast cell lines. Modulation of normal fibroblasts with 0.05, 0.10 and 0.20 micromol/L adrenaline resulted in a statistically significant (P < 0.01) decrease in the expression of I procollagen mRNA. However, only 0.20 micromol/L adrenaline can decreased the mRNA expression of I procollagen in the hypertrophic scar fibroblasts.</p><p><b>CONCLUSIONS</b>We conclude from these results that adrenaline can increase the production of bFGF and decrease production of TGF-beta1 and I procollagen in human normal dermal and hypertrophic scar fibroblasts cultured in vitro.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
RNA, Messenger
/
Epinephrine
/
Cells, Cultured
/
Fibroblast Growth Factor 2
/
Procollagen
/
Cicatrix, Hypertrophic
/
Collagen Type I
/
Transforming Growth Factor beta1
/
Fibroblasts
Type of study:
Prognostic study
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Plastic Surgery
Year:
2005
Type:
Article
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