A three-dimension in vitro model for angiogenesis of hemangioma / 中华整形外科杂志

ABSTRACT

<p><b>OBJECTIVE</b>To create a three dimension (3D) in vitro model for angiogenesis of hemangioma.</p><p><b>METHOD</b>The fragment of hemangioma specimen was embedded in fibrin gel to set up the three-dimension (3D) in vitro model for angiogenesis of hemangioma.</p><p><b>RESULT</b>In the model, microvessels grew out from the tissue fragments at the 2nd to 3rd day after culture, and at the 8th to 9th day a compact network of microvessels come into being, then tending to be stationary. The compact network around the tissue fragment was confirmed to be blood vessels by immunohistochemistry and electron microscopy.</p><p><b>CONCLUSION</b>This model helps to study the mechanism of hemangioma angiogenesis and investigate the drugs of anti-angiogenesis.</p>