Antivirus effect of plasmid co expressing hepatitis B surface antigen and granulocyte macrophage-colony stimulating factor / 中华肝脏病杂志
Chinese Journal of Hepatology
;
(12): 201-204, 2004.
Article
in Chinese
| WPRIM
| ID: wpr-240439
ABSTRACT
<p><b>OBJECTIVE</b>To investigate whether GM-CSF can enhance the antiviral effect of HBsAg DNA vaccine.</p><p><b>METHODS</b>Divided animals into 8 groups. Group A pcDNA3.1-S 100microg; Group B pcDNA3.1-GM-CSF-S 100microg; Group C pcDNA3.1-S-GM-CSF 100microg; Group D pcDNA3.1-S 50microg + pcDNA3.1-GM-CSF 50microg; Group E pcDNA3.1-GM-CSF 100microg; Group F recombinant HBsAg vaccine 1microg; Group G pcDNA3.1,100microg; Group H PBS 100microl. Serum HBsAg level and concentration of IL-2, IL-4 and IFN-gamma were examined using commercial ELISA kit. The [3H] thymidine incorporation into DNA of Spleen cells was measured; HBsAg expression of hepatocytes from HBV-transgenic mice was assessed using immunohistochemical analysis.</p><p><b>RESULTS</b>Serum HBsAg level was lower and concentration of IL-2, IFN-gamma and SI was higher in mice immunized with pcDNA3.1-GM-CSF-S than those from mice immunized with pcDNA3.1-S and other groups (F=11.262, P<0.01, F=8.147, P<0.01, F=62.275, P<0.01, F=116.28, P<0.01. Less Hepatic HBsAg expression and decline of pcDNA3.1-GM-CSF-S of mice immunized with pcDNA3 were observed in comparison with control groups (F=41.439, P<0.01). Liver histological analysis showed no evidence of necrosis or inflammation in various groups.</p><p><b>CONCLUSION</b>The plasmid co expressing GM-CSF and HBsAg could improve HBsAg-specific humoral and cellular immune responses induced by plasmid encoding HBsAg alone and enhance HBsAg DNA vaccine antivirus effect.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Plasmids
/
Mice, Transgenic
/
Lymphocyte Activation
/
Granulocyte-Macrophage Colony-Stimulating Factor
/
Interleukin-4
/
Interferon-gamma
/
Interleukin-2
/
Hepatitis B Vaccines
/
Vaccines, DNA
/
Allergy and Immunology
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Hepatology
Year:
2004
Type:
Article
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