Significance of serological markers and virological marker for hepatitis E in rhesus monkey model / 中华肝脏病杂志
Chinese Journal of Hepatology
; (12): 7-10, 2004.
Article
in Zh
| WPRIM
| ID: wpr-240527
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the serological markers and biological marker in the diagnosis of hepatitis E infection in a rhesus monkey model.</p><p><b>METHODS</b>86 rhesus monkeys had been infected with different doses of HEV. Hence, they were taken sequential blood samples at intervals up to 86 weeks for 4 hepatitis E virus (HEV) specific antibody assays (E2-IgM, E2-IgG, GL-IgG, and YES-IgG), and nucleic acid assay.</p><p><b>RESULTS</b>All the animals produced E2-IgG and all but one also produced E2-IgM and excreted the virus in stool, whereas positive rate of GL-IgG and YES IgG were low and correlated with virus level. Hepatitis occurred over a period of 4 weeks (between 3 an 7 weeks) after infection. Virological marker occurred mainly during incubation period and declined rapidly after onset of hepatitis. Seroconversion of E2-IgM occurred before onset of hepatitis in 70% monkeys and declined rapidly up to 50% of peak value after 4 weeks. E2-IgM seroconversion was closely paralleled by E2-IgG; however, E2-IgG persisted in all animals for the entire duration of experiment of up to 86 weeks. Production of GL-IgG and YES-IgG was delayed by one week after the E2 antibodies, these antibodies showed a transient occurrence and seroprevalence declined to 50% of the peak value over a period of 12 weeks.</p><p><b>CONCLUSION</b>E2-IgM might be used as a suitable acute hepatitis E marker, and E2-IgG as a suitable epidemiological marker. The seroconversion or titer elevation of GL-IgG and YES-IgG antibodies probably used to confirm the infection. The viral markers are optional for early diagnosis.</p>
Full text:
1
Index:
WPRIM
Main subject:
Blood
/
Immunoglobulin E
/
Immunoglobulin M
/
Biomarkers
/
Hepatitis Antibodies
/
Classification
/
Hepatitis E virus
/
Hepatitis E
/
Diagnosis
/
Alanine Transaminase
Type of study:
Diagnostic_studies
/
Screening_studies
Limits:
Animals
Language:
Zh
Journal:
Chinese Journal of Hepatology
Year:
2004
Type:
Article